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How and When to Use Multiple Techniques in Biophysical Protein Characterization

April 01, 2015
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The aggregation of biopharmaceuticals is a complex physical process. Its analysis requires the use of a selection of suitable methods to build up a comprehensive dataset that can then support appropriate interpretation and scientifically sound conclusions.

The orthogonal methods discussed in this white paper (SV-AUC, SEC, SEC-MALS, DLS, SDS-Page and FFF) provide ways of characterizing soluble aggregates but have a number of limitations in their abilities to provide quantitative information. Proper method selection ensures data is both orthogonal and valuable, and that none of the data collected is redundant.


How and When to Use Multiple Techniques

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