In-vitro efficacy testing for fungicides makes multiple testing faster, confirms the results of in-vivo testing and can reproduce a range of environmental factors.


Fungicide efficacy testing, against a range of phytopathogens, is hugely important to the agriculture sector as it tries to mitigate the variable nature of the diseases attacking seeds and crops in the field.

Over the years, application of fungicides has eroded their effectiveness, as diseases adapt and become increasingly resistant to traditional treatments. This means that today, testing is conducted against specific disease strains, and country conditions. Different strains may react differently to the same product – efficacy testing reveals these differences.

In-vitro methods for fungicide efficacy testing are a group of analytical methods which reproduce a range of environmental factors under artificial, laboratory, conditions. Bringing these tests indoors has a range of research benefits, including:

  • Reduction of the factors that influence the results of treatments at the experimental stage
  • Shortest period to obtain primary data – in-vitro testing takes 3–5 days for the full growth of fungus test objects, and 1–2 days for bacteria
  • Reduction in analysis costs
  • Potential to test multiple fungicides and targets simultaneously
  • Confirmation of results obtained under in-vivo conditions

New Methodology

In Ukraine, SGS’s microbiological laboratory has developed a fungicide efficacy testing methodology that allows contemporaneous evaluation of a wide range of treatments against phytopathogens.

The approach we have developed evaluates the efficacy of plant protection treatments against the major groups of phytopathogens, and is based on the classical methods of microbiology and molecular genetics. In addition, the method of cryopreservation applied to microorganisms in our laboratory, allows testing to be conducted throughout the year.

In-vitro testing facilitates the study and evaluation of:

  • Fungicide efficacy against phytopathogens, including territory specific variations
  • Field trial results, in comparison to in-vitro testing
  • The efficacy of treatments with biopesticides at different stages (after processing, under conditions of vegetation)

To ensure the most accurate and effective results, we use molecular genetic methods (based on PCR analysis) to detect and identify phytopathogen pathotypes. We can also conduct microbiological assessment of the risks, in the field.

This method allows large amounts of preliminary research work to be completed in a short period of time. With in-vitro we can test efficacy against numerous diseases, harmful to different crops. As a result, we can then develop the most appropriate field trial schemes. This approach will reduce costs for plant protection product development, significantly so for the most extensive field trials.

For further information, please contact:

Eduard Ershov
Business Manager
Atama Golovatogo 40/42
65003, Odessa
SGS Ukraine
t: +38 048-7869600