Email this pageEmail this pagePrint this pagePrint this page
Share on LinkedInShare on LinkedInShare on FacebookShare on FacebookTweet thisTweet this

Life Sciences

Categories

Minimize menuMaximize menu

Minimize menuMaximize menuHomeLife SciencesLaboratory ServicesProtein & Peptide AnalysisN-Terminal Sequencing

Related Services & Resources

N-Terminal Sequencing

SGS’s protein characterization services are renowned across a wide range of life science companies for their reliability and accuracy. A key element of our service package is N-terminal Sequencing, which determines the amino acid sequence of a peptide or protein.

Using automated Edman Degradation Sequencing, also known as Gas Phase Sequencing, our experts work on samples in solution or from proteins that have been separated using SDS-PAGE and blotted onto PVDF membranes. They:

  • Sequentially break down the peptide or protein into its constituent amino acids from the N-terminus of the sample
  • Derivatize and separate the produced amino acids by reverse-phase high pressure liquid chromatography
  • Visualise the amino acids by UV detection for each cycle of Edman chemistry
  • Quanitify the amino acids by comparison to a standard mixture

In addition to N-Terminal Sequencing we are able to assess possible truncations of the amino-terminus or levels of chemically blocked termini that usually prevent full sequencing. We may be able to remove the blocking group before sequencing.

Life science companies across the globe are using SGS N-terminal sequencing throughout the drug discovery cycle and during manufacturing, to demonstrate comparability and consistency between batches. In addition, N-terminal sequencing can also be used for de novo sequencing of proteins.

Contact SGS today to discover more about how we can help with your (glycol)protein and peptide analysis.

Email Us
Lab ServicesLaboratory Services by SGSA comprehensive look at SGS’s global lab services (PDF 1,4MB) ...
Interactive Pocket MapLife Science Services & Sites Pocket MapA quick reference to our global sites and services. ...
Biopharmaceutical BrochureLab Services Biopharmaceutical Laboratory Services Brochure ...

Contact

SGS Headquarters

  • 1 Place des Alpes
  • P.O. Box 2152
  • Geneva, 1211
  • Switzerland
  • t   +41 22 739 91 11
  • f   +41 22 739 98 86
  • e   Email Us

E-SUBSCRIPTIONS

Sign up to SGS publications and newsletters from your region and around the world
STAY UP TO DATE.SUBSCRIBE NOW

Office Directory

Find SGS offices and labs around the world.World map
Office Locator

News

events