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HomeLife SciencesBiopharmaceutical ServicesLaboratory ServicesProtein & Peptide AnalysisDisulfide Bridge Analysis
The number and position of disulfide bridges (also called disulfide bonds) plays a crucial role in obtaining a correctly folded protein product essential for function. Our disulfide bridge assessment helps you identify the number and position of disulfide bridges present.
Our analytical services help you to:
As the world’s leading provider of disulfide bridge analysis, we offer you the experience, technical and regulatory expertise, and unique global network you need.
We achieve disulfide bridge and free sulfhydryl group assignment using peptide mapping-based strategies. Analysis generally involves digestion using a suitable enzyme and/or chemical and examination of the resulting mixture using Liquid Chromatography with Mass Spectrometry Detection (LC-MS) to isolate and identify potential disulfide bridged peptides.
The potential bridges can then be confirmed by reduction and re-analysis using:
Using such powerful mass spectrometric techniques enables even low levels of potential mismatched or scrambled disulfide bridge forms to be detected.
Contact us today to find out more about our disulfide bridge analysis.